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The synthesis of GSH from its constituent amino acids involves two ATP-requiring enzymatic steps: L-glutamate + L-cysteine + ATP → γ-glutamyl-L-cysteine + ADP + Pi γ-glutamyl-L-cysteine + L-glycine + ATP → GSH + ADP + Pi The first step of GSH biosynthesis is considered rate limiting and catalyzed by glutamate cysteine ligase (GCL, EC 6.3.2.2